By Oscar; Latner, A. L. Bodansky
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20). A strip-chart recorder can also be added to monitor the reaction progress-curves. The AC 1 unit performs the functions of sample dilution, reagent addition, mixing, preincubation, initiation of the enzymic reaction, and transfer of the completed reaction mixtures to the cuvette of the spectrophotometer. The serum or other samples are loaded in disposable cups into racks which also carry the disposable reaction tubes. The capacity of the unit is 10 loaded racks, although more can be added at any time as places become available.
As each sample subsequently enters the light beam its absorbance is determined. This value is recorded as the background absorbance in the form of a peak on the chart. The output of the photocell adjusts the gain of the amplifier during background measurement and zeros the recorder. 05 A full-scale deflection, 26 D. W. 05 A is reached during the measuring interval. 2 A range can be selected initially. The LKB reaction rate analyzer gives results that are well correlated with those by other methods (Fig.
10. The LKB reaction-rate analyzer. The analyzer is shown standing on a cooling stage which allows operation temperatures of 25", 30°, 32", 35", or 37°C to be selected. The strip-chart recorder is not shown. ) set temperature (35" or 37°C; - ~ 0 . 2 " Cis) reached. Lower temperatures can be obtained by standing the apparatus on a cooling stage. At the measuring position a pump (range 25-250 1)1. adds a measured volume of initiating reagent. 5 seconds. Internal vanes in the cuvette aid mixing. 5 seconds, during which the background absorbance is measured and the recorder is zeroed.