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By Motomichi Torisu

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US) 63, 1457-1464. Miller, F. , and Heppner, G. H. (1979b). Proc. Amer. As soc. Cancer Res. 21, 201. Miller, F. , Miller, B. , and Heppner, G. H. (1983). Invasion and Metastasis 3, 22-31. Morahan, P. , Edelson, P. , and Gass, K. (1980). J. Immunol. 125, 1312-1317. Mule, J. , Forstrom, J. , and Hellstrom, K. E. (1981). Int. J. Cancer 28, 611-614. Nathan, C. , Silverstein, S. C , Brukner, L. , and Cohn, Z. A. (1979). J. Exp. Med. 149, 100-113. , and Nicolson, G. L. (1981). Int. J. Cancer 28, 731-738.

1. In a tumor where expansion is by growth, it is expec­ ted that within a three-square microscope eyepiece grid placed at the tumor margin, the top grid would show saturation density of cancer cells, the intermediate grid would show a density between saturation and zero, and the bottom grid would contain no cancer cells. The transition from saturation to zero occurs over three squares. of tissue section as shown in Fig. 1, the square over the tumor proper will contain cancer cells at saturation density, the square over the host tissue proper will contain no cancer cells at all, whereas the intermediate square over the cancer—host tissue inter­ face will contain a variable number of cells progressing from zero to saturation density as the melanoma expands.

8. A new antigen appearing on the cell surface as indi­ cated by granules of immunoferritin. There is a virus budding, and the new foreign antigen (arrows) that is produced on the surface after xenogenization may be recognized as foreign by the host. 3. REGRESSION OF XENOGENIZED TUMOR METASTASIS IN RATS 47 F i g . 9. A s c a n n i n g e l e c t r o n m i c r o g r a p h showing tumor c e l l s w i t h a number of m i c r o v i l l i on t h e s u r f a c e ; t h e r e a r e f i b r o s a r c o m a c e l l s before infection with v i r u s .

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