Download Ciba Foundation Symposium 195 - T Cell Subsets in Infectious by CIBA Foundation Symposium PDF

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T cells are a really good inhabitants of immune cells that reduction the immune method in scuffling with a variety of forms of invading pathogens. This ebook offers up to the moment information at the position of T cells in autoimmune illnesses.

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Flavell: I’ve used the green fluorescent protein as a marker for the IL-4 gene (unpublished results), which allows the analysis of cells without antibody staining. Cells can then be detected by fluorescence microscopy. I am also using cell surface staining with CD2, by directing expression of the CD2 gene with the IFN-y promoter. There is also the issue of promoter specificity, so the ultimate method would be to recombine the gene into the chromosome using the gene targeting. I am now doing these experiments.

Acknowledgement The DNAX Research Institute is supported by the Schenng-Plough Corporation. Reversal of T h l and Th2 populations 25 References Belosevic M, Finbloom DS, van der Meide PH, Slayter MV, Nacy CA 1989 Administration of monoclonal anti-IFN-gamma antibodies in vivo abrogates natural resistance of C3H/HeN mice to infection with Leishmania major. J Immunol 143:26&274 Chatelain R, Varkila K, Coffman RL 1992 IL-4 induces a Th2 response in Leishmania major-infected mice. J Immunol 148:1182-1187 Coffman RL, Varkila K, Scott P, Chatelain R 1991 Role of cytokines in the differentiation of CD4+ T cell subsets in vivo.

This also contrasts with some of the published results in terms of IL-12 potentially being produced by dendritic cells (Macatonia et a1 1995). Abbas: It may have profound effects on APCs, but I’m asking a more limited question. Is the ability of IL-4 or IL-12 to drive Th2 cell or Thl cell differentiation due to the effects on APCs or the effects on T cells? Lotze: It depends on where you look. Most of our systems involve culturing dendritic cells under various cytokine conditions and then giving them peptides.

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